The mapping population used in this study (A05141) consisted of 162 F1 progeny derived from a cross between ‘Rio Grande Russet’ (female parent) and ‘Premier Russet’ (male parent). ‘Premier Russet’ is a dual purpose (processing and fresh market) potato variety with significant resistance to the accumulation of reducing sugars after long-term storage at low temperatures . Rio Grande Russet is a high yielding fresh market potato variety . A05141 was developed at the USDA-ARS Small Grains and Potato Germplasm Research Unit (Aberdeen, ID) and was one of the mapping populations phenotyped and genotyped by the Solanaceae Coordinated Agricultural Project (SolCAP) .
Industry tests was in fact held from the USDA-ARS, College or university away from Idaho Lookup and you may Extension Center (Aberdeen, ID) and you will Vermont County College or university Tidewater Research Route (Plymouth, NC) in the summers out of 2010 and you will 2011. The brand new progeny and mothers were analyzed when you look at the a great randomized done take off framework having several replications out-of ten-slope plots at each location. Vine readiness was aesthetically obtained on the a-1–nine measure during the 95 (95DAP) and you can 120 (120DAP) weeks immediately after growing, having 1 = early, 100% vines during the a storyline dry, if you’re nine = really late, complete bloom with buds apparent. Bush increases routine was filed early in flowering and you may expressed while the step 3 = erect (> 45° that have surface), 5 = semi-upright (30–45° which have ground), and you will seven = distribute (More file 10: Dining table S5). Verticillium wilt and very early blight (EB) (Altenaria tenuis) disease were rated toward a great 0–nine size. For EB, 0 indicates no disease and you can nine > 90% of your leaf area affected inside a storyline. For VW, the dimensions is a combined rating having leaf urban area/stem flagging considering the condition (Most file ten: Desk S5). Plots weren’t inoculated, but Aberdeen industries possess large inoculum away from Verticillium on the soil, built up more decades. Very early blight sometimes appears yearly from the ID and you can NC places as well as so it demo the condition are permitted to write obviously, and no fungicides applied for their control.
Tuber trials was indeed treated with Isopropyl Chlorpropham (N-(3-chlorophenyl) carbamate) at a rate away from twenty two ppm to avoid sprouting and stored in the 42 °F and 95% cousin dampness getting ninety days.
Sugar concentrations escort services Arvada to the services inside trial had been determined away from good ten-tuber attempt by using the sorts of Sowokinos mais aussi al. having changes. Tubers was indeed reduce using a passionate Kut Footwear Stringer French fry cutter. Two hundred grams of tuber structure collected on the cardiovascular system of the brand new 10 tubers have been macerated for the an enthusiastic Acme Juicerator (Acme Devices, Springtime Mountain, FL). Through the processing, tuber structure try washed with 150 mL out of salt-phosphate buffer (0.05 Yards, pH eight.5) getting a last homogenate level of 275 mL. Glucose concentrations were determined playing with a great YSI design 2700 Analyzer (Purple Springs Means Co., Inc., Red Springs, OH) and shown with the a percent new lbs foundation.
A remove regarding structure (step three.0 cm ? 0.8 cm) taken from each one of the ten tubers utilized in the newest glucose extraction procedure was utilized to possess fry colour determination (10 strips each imitate). Strips was in fact deep-fried in the canola oil on 191 °C to possess step three.5 min. Fry color are computed in this step 3 min having fun with a design 577 Photovolt Reflection Meter (model 577, Photovolt Tool Inc., Minneapolis, MN). A green filter was applied as well as the device is actually calibrated using a black-cavity simple since the 0.0% reflectance and a white plaque (Pet. No. 26-5Cat. No. 26–570-08) while the 99.9% reflectance. Specifications was started brand new ends up of any remove. The lower the latest reflectance dimension, the newest dark the new fry colour and you can vice versa.